![]() ![]() ![]() QIAGEN MiniPrep Plasmid isolation kit (Qiagen Inc., Germany) is used for plasmid extraction. Fifty colonies were randomly selected for further analysis. The clones were grown in LB medium plates, supplemented with ampicillin (100 m g/ml) and were incubated overnight at 37 C. Ten ng/ m l of genomic DNA was added as a template in a total reaction volume of 50 m L. PCR reaction was set up as described previously. 2004) targeting a large fragment of the 16S rRNA gene were used for PCR prior to clone library analysis. Primers named Arch7f and Arch1384r (Lueders et al. gene for Sample 1- 148bac was constructed using TOPO TA Cloning Kit for Sequencing (Invitrogen, Carlsbad, USA) as described in the manufacturer ’ s instructions. ![]()
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